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cd11b 149sm  (fluidigm)


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    Structured Review

    fluidigm cd11b 149sm
    Cd11b 149sm, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd11b+149sm/Anti-Human+CD194%2FCCR4+(L291H4)-149Sm/pmc11490492__41467_2024_53262_MOESM3_ESM-80-56-57
    Average 93 stars, based on 12 article reviews
    cd11b 149sm - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Biomarker Discovery:

    Article Title: Spatial dynamics of CD39 + CD8 + exhausted T cell reveal tertiary lymphoid structures-mediated response to PD-1 blockade in esophageal cancer
    Article Snippet: The following anti-human primary antibodies for multicolor flow cytometry were used: Ki-67-BV421 and Ki-67-PE/Cy7 (BioLegend, Clone Ki67, Cat No. 350506, 350526, 1:50), CD4-PerCP/Cy5.5 (BioLegend, Clone SK3, Cat No. 344608, 1:50), CD39-APC/Fire750 and CD39-BV421 (BioLegend, Clone A1, Cat No. 328230, 328214, 1:20, 1:25), Eomes-FITC (eBioscience, Clone WD1928, Cat No. 11-4877-42, 1:20), Tox-PE (eBioscience, Clone TXRX10, Cat No. 12-6502-82, 1:20), Tbet-PE/Cy7 (BioLegend, Clone 4B10, Cat No. 644824, 1:50), CD14-BV510 (BioLegend, Clone M5E2, Cat No. 301842, 1:50), CD8-BV605 (BioLegend, Clone SK1, Cat No. 344742, 1:50), TIM-3-BV711 (BD, Clone 7D3, Cat No. 565567, 1:50), CD69-FITC (BioLegend, Clone FN50, Cat No. 310904, 1:20), TCF-1-PE (BioLegend, Clone 7F11A10, Cat No. 655208, 1:20), OX-40-BV421 (BioLegend, Clone ACT35, Cat No. 350014, 1:50), IFN--BV711 (BD, Clone 4S.B3, Cat No. 564039, 1:50), TNF-FITC (BD, Clone Mab11, Cat No. 554512, 1:50), Mouse IgG1, Isotype Ctrl-BV421 (BioLegend, Cat No. 400157), Mouse IgG1, Isotype Ctrl-PE (BioLegend, Cat No. 400111), Mouse IgG1, Isotype Ctrl-FITC (BioLegend, Cat No. 400107), Mouse IgG1, Isotype Ctrl-PE/Cy7 (BioLegend, Cat No. 400125), Rat IgG2a, Isotype Ctrl-PE (BD, Cat No. 553930), and Mouse IgG1, Isotype Ctrl-BV711 (BioLegend, Cat No. 400167). .. The following primary antibodies were used for IMC: SMA-141Pr (Fluidigm, Clone 1A4, Cat No. 3141017D, 1:400), Vimentin-143Nd (Fluidigm, Clone D21H3, Cat No. 3143027D, 1:1600), CD19-142Nd (Fluidigm, Clone 6OMP31, Cat No. 3142014D, 1:200), CD14-144Nd (Fluidigm, Clone EPR3653, Cat No. 3144025D, 1:1600), CD163-147Sm (Fluidigm, Clone EDHu-1, Cat No. 3147021D, 1:400), PanKeratin-148Nd (Fluidigm, Clone C11, Cat No. 3148020D, 1:200), CD11b-149Sm (Fluidigm, Clone L291H4, Cat No. 3149030D, 1:100), PD-L1-150Nd (Fluidigm, Clone SP142, Cat No. 3150033D, 1:50), OX40-151Eu (Fluidigm, Polyclonal, Cat No. 3151024D, 1:100), CD45-152Sm (Fluidigm, Clone D9M8I, Cat No. 3152018D, 1:1600), CD45-152Sm (Fluidigm, Clone CD45-2B11, Cat No. 3152016D, 1:100), LAG3-153Eu (Fluidigm, Clone D2G4O, Cat No. 3153028D, 1:200), TIM-3-154Sm (Fluidigm, Clone D5D5R, Cat No. 3154024D, 1:800), FoxP3-155Gd (Fluidigm, Clone 236A/E7, Cat No. 3155016D, 1:50), CD4-156Gd (Fluidigm, Clone EPR6855, Cat No. 3156033D, 1:200), E-cadherin-158Gd (Fluidigm, Clone 24E10, Cat No. 3158029D, 1:400), CD68-159Tb (Fluidigm, Clone KP1, Cat No. 3159035D, 4 nature portfolio | reporting sum m ary April2023 Validation Novel plant genotypes Seed stocks Authentication Plants 1:1600), CD20-161Dy (Fluidigm, Clone H1, Cat No. 3161029D, 1:800), CD8a-162Dy (Fluidigm, Clone C8/144B, Cat No. 3162034D, 1:800), TCF-1-163Dy (CST, Clone C63D9, Cat No. #85942, 1:100), Tox-164Dy (CST, Clone E613Q, Cat No. #62886, 1:400), PD-1-165Ho (CST, Clone D4W2J, Cat No. 63815SF, 1:100), CD45RA-166Er (Fluidigm, Clone HI100, Cat No. 3166031D, 1:1600), CD69-167Er (Abcam, Clone EPR21814, Cat No. ab234512, 1:200), Ki-67-168Er (Fluidigm, Clone B56, Cat No. 3168022D, 1:200), Collagen I-169Tm (Fluidigm, Polyclonal, Cat No. 3169023D, 1:200), CD3-170Er (Fluidigm, Polyclonal, Cat No. 3170019D, 1:100), CD11c-171Yb (CST, Clone D3V1E, Cat No. #93233, 1:200), CD39-172Yb (Abcam, Clone EPR20627, Cat No. ab236038, 1:50), CD45RO-173Yb (Fluidigm, Clone UCHL1, Cat No. 3173016D, 1:3200), CD28-173Yb (Abcam, Clone ERP22076, Cat No. ab243557, 1:50), HLA-DR-174Yb (Fluidigm, Clone LN3, Cat No. 3174025D, 1:1600), CD25-175Lu (Fluidigm, Clone EPR6452, Cat No. 3175036D, 1:50), ICSK1-195Pt (Fluidigm, Cat No. 201500, 1:100), ICSK2-196Pt (Fluidigm, Cat No. 201500, 1:100), ICSK3-198Pt (Fluidigm, Cat No. 201500, 1:100). .. The following primary antibodies and detection systems were used for critical IHC assays: PD-L1 antibody (28-8) IHC antigen retrieval and detection panel (Abcam, Clone 28-8, Cat No. ab236676, 1:50), Anti-PD-1 (Abcam, Clone NAT105, Cat No. ab52587, 1:50), AntiCD39 (Abcam, Clone ERP20627, Cat No. ab223842, 1:500), Anti-CD8 (Leica Biosystems, Clone 4B11, Cat No. PA0183, Prediluted), Goat anti-mouse IgG2b CF Dye 647 (Biotium, Polyclonal, Cat No. 20272-1, 1:800), Goat anti-mouse IgG1 CF Dye 488 (Biotium, Polyclonal, Cat No. 20246-1, 1:800), Goat anti-rabbit IgG CF Dye 568 (Biotium, Polyclonal, Cat No. 20103-1, 1:800), EnVision+ Dual Link System-HRP (DAB+) (Agilent Technologies, Cat No. K406311, Prediluted), Anti-CD3 (Agilent Technologies, Clone F7.2.38, Cat No. M7254, 1:20), Anti-CD20cy (Agilent Technologies, Clone L26, Cat No. IS604, Prediluted), and Anti-PNA (Novus, Clone MECA79R, Cat No. NBP2-78792, 1:100).



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    fluidigm anti hu ms cd11b epr1344 146nd
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    Image Search Results


    Inhibition of Drp1 by Mdivi-1 alleviates IMQ-induced skin inflammation (A) Treatment schema. WT mice were applied daily with topical IMQ cream for 5 days and received Mdivi-1 or DMSO (50 mg/kg, i.p.) treatment for 6 days. (B) Representative gross images of back skin are shown. (C) Representative H&E-stained skin sections are shown (n = 11–12/each group). Epidermal thickness at day 6 were measured. Scale bar, 100um. (D and E) Skin single-cell suspensions were stained for CD11b/Gr-1 (D) and IL-17 production (E, top) without stimulation in γδ T cells (n = 7/each group). Cells from draining LN were stained for IL-17 production after stimulated with PMA/I plus Golgi-Plug for 5 h (E, bottom). Data are representative of two independent experiments with similar results. Data are shown as mean ± SEM. Unpaired Student’s t test was used. ∗p < 0.05, ∗∗p < 0.01. (F) Representative IMC images of skin sections stained with metal isotope labeled Abs against CD11b (red), F4/80 (green), and DNA (blue). Scale bars, 100um and 50um in magnification. (G) UMAP analysis of the skin IMC images from DMSO or Mdivi-1 treated mice.

    Journal: iScience

    Article Title: Natural γδT17 cell development and functional acquisition is governed by the mTORC2- c -Maf-controlled mitochondrial fission pathway

    doi: 10.1016/j.isci.2023.106630

    Figure Lengend Snippet: Inhibition of Drp1 by Mdivi-1 alleviates IMQ-induced skin inflammation (A) Treatment schema. WT mice were applied daily with topical IMQ cream for 5 days and received Mdivi-1 or DMSO (50 mg/kg, i.p.) treatment for 6 days. (B) Representative gross images of back skin are shown. (C) Representative H&E-stained skin sections are shown (n = 11–12/each group). Epidermal thickness at day 6 were measured. Scale bar, 100um. (D and E) Skin single-cell suspensions were stained for CD11b/Gr-1 (D) and IL-17 production (E, top) without stimulation in γδ T cells (n = 7/each group). Cells from draining LN were stained for IL-17 production after stimulated with PMA/I plus Golgi-Plug for 5 h (E, bottom). Data are representative of two independent experiments with similar results. Data are shown as mean ± SEM. Unpaired Student’s t test was used. ∗p < 0.05, ∗∗p < 0.01. (F) Representative IMC images of skin sections stained with metal isotope labeled Abs against CD11b (red), F4/80 (green), and DNA (blue). Scale bars, 100um and 50um in magnification. (G) UMAP analysis of the skin IMC images from DMSO or Mdivi-1 treated mice.

    Article Snippet: Antibodies were purchased from Fluidigm and concentration were used for anti-Hu/Ms CD11b (EPR1344)-146Nd (91H007146) at 1:100; anti-F4/80 (D2S9R)-166Er (91H030166) at 1:100; anti-Mouse Ly-6G (1A8)-141Pr (3141008B) at 1:50.

    Techniques: Inhibition, Cream, Staining, Labeling

    Journal: iScience

    Article Title: Natural γδT17 cell development and functional acquisition is governed by the mTORC2- c -Maf-controlled mitochondrial fission pathway

    doi: 10.1016/j.isci.2023.106630

    Figure Lengend Snippet:

    Article Snippet: Antibodies were purchased from Fluidigm and concentration were used for anti-Hu/Ms CD11b (EPR1344)-146Nd (91H007146) at 1:100; anti-F4/80 (D2S9R)-166Er (91H030166) at 1:100; anti-Mouse Ly-6G (1A8)-141Pr (3141008B) at 1:50.

    Techniques: Purification, Virus, Recombinant, Saline, Staining, Cell Isolation, Software